User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/10: Difference between revisions

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==Summary==
==Summary==
* GAPDH western blot VASP blot
* GAPDH western blot VASP blot (1:2000) --> Ht4 cells unsuccesfull, repeat 1:500 dilution
* Start stimulation D9
* Start stimulation D9
* Split other D9 hTERT
* Split other D9 hTERT

Revision as of 06:02, 10 March 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • GAPDH western blot VASP blot (1:2000) --> Ht4 cells unsuccesfull, repeat 1:500 dilution
  • Start stimulation D9
  • Split other D9 hTERT


  • Stop stimulation D12
  • Split Ht4
    • collect cells in end volume 21 mL
    • Take 7 mL of cell suspension and dilute in 53 mL
    • Divide over 4 flask (15 mL each)

Materials & Methods

Materials

Method

6-wells
# 1 2 3
A
B
24-wells
# 1 2 3 4 5 6
A
B
C
D
96-wells plate
# 01 02 03 04 05 06 07 08 09 10 11 12
A
B
C
D
E
F
G
H
I

Results

  • WHAT?

Conclusion

  • DONE Ow..