User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/10: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Start stimulation D9, Stop stimulation D12</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Summary== | ==Summary== | ||
* | * GAPDH western blot VASP blot (1:2000) --> Ht4 cells unsuccesfull, repeat 1:500 dilution --> still unsuccesfull | ||
* Start stimulation D9 | |||
* Split other D9 hTERT | |||
<br> | |||
* Stop stimulation D12 | |||
* Split Ht4 | |||
** collect cells in end volume 21 mL | |||
** Take 7 mL of cell suspension and dilute in 53 mL | |||
** Divide over 4 flask (15 mL each) | |||
== | |||
==Notes== | |||
* Stop stimulation D12 @ 15.20 | |||
* Stimulation D9 was finished @ 11.30 | |||
{| border="1" | {| border="1" | ||
|+''' | |+'''BLOTS''' | ||
| | | | ||
|D9 Esther | |||
| | | | ||
|- | |- | ||
|P65 IV | |||
| | | | ||
| | |P65 V | ||
|- | |- | ||
|HT4 VASP | |||
| | |||
| | | | ||
|D9 VASP | |||
|- | |- | ||
|} | |} | ||
==Results== | ==Results== | ||
* | * [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/16#Attachment| Viability staining]] | ||
*PICTURE --> ANOUK | |||
==Conclusion== | ==Conclusion== | ||
*{{done}} Ow.. | *{{done}} Ow.. | ||
==Same topics== | |||
* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/10|Splitting cells 10Feb2010]] | |||
* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/09|Splitting cells 09March2010]] | |||
Revision as of 02:52, 23 March 2010
<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>
 Start stimulation D9, Stop stimulation D12
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Summary
Notes
Results
Conclusion
Same topics
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