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| ** Divide over 4 flask (15 mL each) | | ** Divide over 4 flask (15 mL each) |
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| ==Materials & Methods==
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| ===Materials===
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| ===Method===
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| {| border="1"
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| |+'''6-wells'''
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| {| border="1"
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| |+'''24-wells'''
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| !#
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| !<u>3</u>
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| !<u>4</u>
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| !<u>5</u>
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| |-
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| |}
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| {| border="1"
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| |+'''96-wells plate'''
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| |#
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| !01
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| !02
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| !E
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| !F
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| !H
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| |}
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| ==Notes== | | ==Notes== |
Revision as of 02:52, 23 March 2010
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Start stimulation D9, Stop stimulation D12
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
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Summary
- GAPDH western blot VASP blot (1:2000) --> Ht4 cells unsuccesfull, repeat 1:500 dilution --> still unsuccesfull
- Start stimulation D9
- Split other D9 hTERT
- Stop stimulation D12
- Split Ht4
- collect cells in end volume 21 mL
- Take 7 mL of cell suspension and dilute in 53 mL
- Divide over 4 flask (15 mL each)
Notes
- Stop stimulation D12 @ 15.20
- Stimulation D9 was finished @ 11.30
BLOTS
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D9 Esther
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P65 IV
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P65 V
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HT4 VASP
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D9 VASP
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Results
Conclusion
Same topics
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