User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/10

From OpenWetWare
Jump to navigationJump to search

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Start stimulation D9, Stop stimulation D12 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • GAPDH western blot VASP blot (1:2000) --> Ht4 cells unsuccesfull, repeat 1:500 dilution --> still unsuccesfull
  • Start stimulation D9
  • Split other D9 hTERT


  • Stop stimulation D12
  • Split Ht4
    • collect cells in end volume 21 mL
    • Take 7 mL of cell suspension and dilute in 53 mL
    • Divide over 4 flask (15 mL each)

Materials & Methods

Materials

Method

6-wells
# 1 2 3
A
B
24-wells
# 1 2 3 4 5 6
A
B
C
D
96-wells plate
# 01 02 03 04 05 06 07 08 09 10 11 12
A
B
C
D
E
F
G
H
I

Notes

  • Stop stimulation D12 @ 15.20
  • Stimulation D9 was finished @ 11.30
BLOTS
D9 Esther
P65 IV P65 V
HT4 VASP D9 VASP


Results

Conclusion

  • DONE Ow..

Same topics



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/10&oldid=401351"