User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/10

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Start stimulation D9, Stop stimulation D12 Main project page
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Summary

  • GAPDH western blot VASP blot (1:2000) --> Ht4 cells unsuccesfull, repeat 1:500 dilution --> still unsuccesfull
  • Start stimulation D9
  • Split other D9 hTERT


  • Stop stimulation D12
  • Split Ht4
    • collect cells in end volume 21 mL
    • Take 7 mL of cell suspension and dilute in 53 mL
    • Divide over 4 flask (15 mL each)


Notes

  • Stop stimulation D12 @ 15.20
  • Stimulation D9 was finished @ 11.30
BLOTS
D9 Esther
P65 IV P65 V
HT4 VASP D9 VASP


Results

Conclusion

  • DONE Ow..

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