User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/11: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Stop stimulation</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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** 30 μL sample for 25Feb2010 samples | ** 30 μL sample for 25Feb2010 samples | ||
** 15 μL marker | ** 15 μL marker | ||
==Notes== | |||
* SDS PAGE failed after 3 samples of 25Feb2010 & all 8 samples of 05March2010 were put to gel, due to leakage of samples that mixed them | |||
** probable cause is stacking gel that hadn't solidified sufficient, perhaps due to APS 10% that had been prepared earlier that day by someone else and that hadn't been stored on ice. However APS is preservable for 1 week @ -20 °C, so half a day @ RT should be possible. | |||
==Results== | ==Results== | ||
* | *GAPDH--> failure, picture Anouk? | ||
* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/16#Attachment| Viability staining]] | |||
GAPDH--> failure, picture Anouk? | |||
==Conclusion== | ==Conclusion== | ||
*{{done}} Ow.. | *{{done}} Ow.. | ||
===Related entries=== | |||
====Co-IP==== | |||
* | |||
====Run 3: Ht31/S<sup>0</sup>==== | |||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/05|03March2010 Counting cells D9 (P21)/D12 (P25)]] | |||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/09|09March2010 Putting to S<sup>0</sup> D9 (P21)]] | |||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/10|10March2010 Stimulating cells D9 (P21)]] | |||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/11|11March2010 Ending stimulation & Viability D9 (P21)]] | |||
Revision as of 02:53, 23 March 2010
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Stop stimulation | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Summary
Materials & MethodsMaterialsMethod
Notes
Results
Conclusion
Related entriesCo-IPRun 3: Ht31/S0
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