User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/12: Difference between revisions

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==Materials & Methods==
==Materials & Methods==
===Materials===
===Materials===
*  
*DMEM S<sup>+</sup>
** Remove 50 mL of DMEM out of 500 mL
** Add 50 mL FBS
** 3 mL Fungizone
** 10 mL PenStrep
*hTERT Cells
** D12 P26 (9Feb2010)
** D9 P22 (9Feb2010)


===Method===
===Method===

Revision as of 08:34, 15 March 2010

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Summary

  • HT4 cells split 10Feb2010 were stored @ -80°C by Anouk
  • Splitting hTERT
  • Refreshing media

Materials & Methods

Materials

  • DMEM S+
    • Remove 50 mL of DMEM out of 500 mL
    • Add 50 mL FBS
    • 3 mL Fungizone
    • 10 mL PenStrep
  • hTERT Cells
    • D12 P26 (9Feb2010)
    • D9 P22 (9Feb2010)

Method

6-wells
# 1 2 3
A
B
24-wells
# 1 2 3 4 5 6
A
B
C
D
96-wells plate
# 01 02 03 04 05 06 07 08 09 10 11 12
A
B
C
D
E
F
G
H
I

Results

  • WHAT?

Conclusion

  • DONE Ow..