User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/12: Difference between revisions

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===Method===
===Method===
{| border="1"
* Wash cells twice with warm PBS
|+'''6-wells'''
* Add 1 mL Trypsin
!#
* Incubate @ 37 °C
!<u>1</u>
* Collect cells in total volume of 30 mL DMEM S<sup>+</sup>
!<u>2</u>
* Count cells
!<u>3</u>
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|+'''24-wells'''
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|+'''96-wells plate'''
|#
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==Results==
==Results==

Revision as of 08:36, 15 March 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • HT4 cells split 10Feb2010 were stored @ -80°C by Anouk
  • Splitting hTERT
  • Refreshing media

Materials & Methods

Materials

  • DMEM S+
    • Remove 50 mL of DMEM out of 500 mL
    • Add 50 mL FBS
    • 3 mL Fungizone
    • 10 mL PenStrep
  • hTERT Cells
    • D12 P26 (9Feb2010)
    • D9 P22 (9Feb2010)

Method

  • Wash cells twice with warm PBS
  • Add 1 mL Trypsin
  • Incubate @ 37 °C
  • Collect cells in total volume of 30 mL DMEM S+
  • Count cells

Results

  • WHAT?

Conclusion

  • DONE Ow..



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