User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/12: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Splitting & Counting cells</span> | ||
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==Summary== | ==Summary== | ||
* | * HT4 cells split 10Feb2010 were stored @ -80°C by Anouk | ||
* Splitting hTERT | |||
* Refreshing media | |||
==Materials & Methods== | ==Materials & Methods== | ||
===Materials=== | ===Materials=== | ||
* | *DMEM S<sup>+</sup> | ||
** Remove 50 mL of DMEM out of 500 mL | |||
** Add 50 mL FBS | |||
** 3 mL Fungizone | |||
** 10 mL PenStrep | |||
*hTERT Cells | |||
** D12 P26 (9Feb2010) | |||
** D9 P22 (9Feb2010) | |||
===Method=== | ===Method=== | ||
* Wash cells twice with warm PBS | |||
* Add 1 mL Trypsin | |||
* Incubate @ 37 °C | |||
* Collect cells in total volume of 30 mL DMEM S<sup>+</sup> | |||
* Count cells | |||
==Results== | ==Results== | ||
* | *D12 (66×10<sup>4</sup> cells / mL) | ||
**74 | |||
== | **67 | ||
**69 | |||
**54 | |||
* For 24-wells 1.36 mL cells in 11.14 mL S<sup>+</sup>, for 3 petridishes 4.55 mL cells in 25.45 mL S<sup>+</sup> | |||
*D9 (75×10<sup>4</sup> cells / mL) | |||
**74 | |||
**58 | |||
**77 | |||
**64 | |||
* For 24-wells 1 mL cells in 12.5 mL S<sup>+</sup>, for 3 petridishes 4 mL cells in 26 mL S<sup>+</sup> | |||
==Related== | |||
{{todo}} | |||
*Splitting cells | |||
*Counting cells | |||
*History cells | |||
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Revision as of 08:41, 15 March 2010
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Summary
Materials & MethodsMaterials
Method
Results
RelatedTODO
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