User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/17: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Ht31P toxicity</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Summary== | ==Summary== | ||
* Stop stimulation | * Stop stimulation | ||
* Prepare washing buffer 1x ELISA | * Prepare washing buffer 1x ELISA + 10x PBS | ||
* CoIP | * CoIP | ||
==Materials & Methods== | ==Materials & Methods== | ||
===Method=== | ===Method=== | ||
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==Results== | ==Results== | ||
*{{todo| | *{{todo|PICTURES OF CELL DEATH (Anouk)}} | ||
* Viability staining | |||
{| {{table}} | |||
| | |||
| align="center" style="background:#f0f0f0;"|Ht31P (μM) | |||
| align="center" style="background:#f0f0f0;"|Ht31P (μM) | |||
| align="center" style="background:#f0f0f0;"|Ht31P (μM) | |||
| align="center" style="background:#f0f0f0;"|Ht31P (μM) | |||
| align="center" style="background:#f0f0f0;"|Ht31P (μM) | |||
| align="center" style="background:#f0f0f0;"|Ht31P (μM) | |||
|- | |||
| D9 P23 | |||
| align="center" style="background:#f0f0f0;"|'''0''' | |||
| align="center" style="background:#f0f0f0;"|'''10''' | |||
| align="center" style="background:#f0f0f0;"|'''20''' | |||
| align="center" style="background:#f0f0f0;"|'''30''' | |||
| align="center" style="background:#f0f0f0;"|'''40''' | |||
| align="center" style="background:#f0f0f0;"|'''50''' | |||
|- | |||
| align="center" style="background:#C6C6C6;"|'''CTR''' | |||
|7524||4117||2092||1929||1346||1712 | |||
|- | |||
| align="center" style="background:#C6C6C6;"|'''CTR''' | |||
|6719||3030||1540||1323||1126||1458 | |||
|- | |||
| align="center" style="background:#C6C6C6;"|'''CSE''' | |||
|3996||922||649||547||527||660 | |||
|- | |||
| align="center" style="background:#C6C6C6;"|'''CSE''' | |||
|3509||1125||646||608||550||577 | |||
|- | |||
|} | |||
[[Image:SurvivalRateD9P2317032010.JPG]] | |||
<BR> | |||
{| {{table}} | |||
| | |||
| align="center" style="background:#f0f0f0;"|Ht31P (μM) | |||
| align="center" style="background:#f0f0f0;"|Ht31P (μM) | |||
| align="center" style="background:#f0f0f0;"|Ht31P (μM) | |||
| align="center" style="background:#f0f0f0;"|Ht31P (μM) | |||
| align="center" style="background:#f0f0f0;"|Ht31P (μM) | |||
| align="center" style="background:#f0f0f0;"|Ht31P (μM) | |||
|- | |||
| D12 P27 | |||
| align="center" style="background:#f0f0f0;"|'''0''' | |||
| align="center" style="background:#f0f0f0;"|'''10''' | |||
| align="center" style="background:#f0f0f0;"|'''20''' | |||
| align="center" style="background:#f0f0f0;"|'''30''' | |||
| align="center" style="background:#f0f0f0;"|'''40''' | |||
| align="center" style="background:#f0f0f0;"|'''50''' | |||
|- | |||
| align="center" style="background:#C6C6C6;"|'''CTR''' | |||
|7151||5242||3640||2953||2435||2331 | |||
|- | |||
| align="center" style="background:#C6C6C6;"|'''CTR''' | |||
|6816||4353||2746||2339||1970||2074 | |||
|- | |||
| align="center" style="background:#C6C6C6;"|'''CSE''' | |||
|5284||1784||1234||939||938||918 | |||
|- | |||
| align="center" style="background:#C6C6C6;"|'''CSE''' | |||
|4858||1891||1250||1020||870||907 | |||
|- | |||
|} | |||
[[Image:SurvivalRateD12P2717032010.JPG]] | |||
==Conclusion== | ==Conclusion== | ||
* | *Ht31P is lethal for airway smooth muscle hTERT cells | ||
*Strength of effect is donor dependent | |||
Revision as of 07:11, 17 March 2010
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Ht31P toxicity | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Summary
Materials & MethodsMethodPreparations for ELISA & Viability staining
Co-immunoprecipitation Part III
* Be careful not to touch beads Results
Conclusion
|