User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/17

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Summary

  • Stop stimulation
  • Prepare washing buffer 1x ELISA
  • CoIP

Materials & Methods

Materials

Method

Preparations for ELISA & Viability staining

Co-immunoprecipitation Part III

  • Centrifuge sample incubated with beads, 5 min. 14.000 RPM
  • Remove supernatant* (w. syringe)
  • Add 1 mL PBS
  • Centrifuge, 5 min. 14.000 RPM
  • Remove supernatant*
  • Add 75 μL Laemli buffer to beads
  • Store samples @ -20 °C


* Be careful not to touch beads

Results

  • WHAT?

Conclusion

  • DONE Ow..



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