User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/19: Difference between revisions
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| ||50||65||61||73||62,25||622500 | | ||50||65||61||73||62,25||622500 | ||
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<BR> | |||
* Preparing cell dilutions | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''D12''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''24-wells''' | |||
| align="center" style="background:#f0f0f0;"|'''10 cm''' | |||
| align="center" style="background:#f0f0f0;"|'''6-wells''' | |||
| align="center" style="background:#f0f0f0;"|'''15 cm''' | |||
|- | |||
| Cells||585000||625000||1500000||1400000||6000000 | |||
|- | |||
| align="center" style="background:#f00000;"| mL Cells | |||
| align="center" style="background:#f00000;"|1 | |||
| align="center" style="background:#f00000;"|1,07 | |||
| align="center" style="background:#f00000;"|2,56 | |||
| align="center" style="background:#f00000;"|2,39 | |||
| align="center" style="background:#f00000;"|10,26 | |||
|- | |||
| align="center" style="background:#f00000;"| mL S+|| | |||
| align="center" style="background:#f00000;"|11,43 | |||
| align="center" style="background:#f00000;"|17,44 | |||
| align="center" style="background:#f00000;"|11,61 | |||
| align="center" style="background:#f00000;"|34,74 | |||
|- | |||
| SUM||1,00||12,50||20,00||14,00||45,00 | |||
|- | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''D9''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''24-wells''' | |||
| align="center" style="background:#f0f0f0;"|'''10 cm''' | |||
| align="center" style="background:#f0f0f0;"|'''6-wells''' | |||
| align="center" style="background:#f0f0f0;"|'''15 cm''' | |||
|- | |||
| Cells||622500||625000||1500000||1400000||3000000 | |||
|- | |||
| align="center" style="background:#f00000;"| mL Cells | |||
| align="center" style="background:#f00000;"|1 | |||
| align="center" style="background:#f00000;"|1,00 | |||
| align="center" style="background:#f00000;"|2,41 | |||
| align="center" style="background:#f00000;"|2,25 | |||
| align="center" style="background:#f00000;"|4,82 | |||
|- | |||
| align="center" style="background:#f00000;"| mL S+ | |||
|| | |||
| align="center" style="background:#f00000;"|11,50 | |||
| align="center" style="background:#f00000;"|17,59 | |||
| align="center" style="background:#f00000;"|11,75 | |||
| align="center" style="background:#f00000;"|40,18 | |||
|- | |||
| SUM||1,00||12,50||20,00||14,00||45,00 | |||
|- | |- | ||
|} | |} | ||
* Because the total volume was 30 mL, 3x 10.26 mL was not possible, instead ~7 mL was used. | |||
* | |||
==Related entries== | |||
===Run 5: Ht31/S<sup>0</sup> D9/D12=== | |||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/16|Splitting cells 16March2010]] | *[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/16|Splitting cells 16March2010]] | ||
===Same actions=== | |||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/15| Preparation of Medium]] | *[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/15| Preparation of Medium]] | ||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/10| Splitting cells]] | *[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/10| Splitting cells]] |
Revision as of 06:04, 19 March 2010
<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>
Counting hTERT cells | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
SummaryTo prepare for Co-IP, stimulation for ELISA, VASP and to keep the line alive cells were counted and split. Also cells were plated out for Anouk and cells were kept for Wolter to split for his own projects. Materials & MethodsMaterials
MethodPreparation of MediumDMEM S+
Collecting cells
Cell counting
Results
Related entriesRun 5: Ht31/S0 D9/D12Same actions |