User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/19

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Summary

  • Split cells
    • GIVE WOLTER 3×106 cells


  • D9
    • 1x 24-wells (25.000 cells/well (500 μL/well), 625000 cells/12.5 mL)
    • 2x Ø 10cm (750.000 cells/plate (10 mL/plate), 1500000 cells/20 mL)
    • 1x 6-wells (200.000 cells/well (2 mL/well), 1400000 cells/14 mL)
    • 8x Ø 15cm (1×106 cells/plate (15mL/plate), 3x 3×106 cells/45 mL)


  • D12
    • 1x 24-wells (25.000 cells/well (500 μL/well), 625.000 cells/12.5 mL)
    • 2x Ø 10cm (750.000 cells/plate (10 mL/plate), 1.500.000 cells/20 mL)
    • 1x 6-wells (200.000 cells/well (2 mL/well), 1.400.000 cells/14 mL)
    • 8x Ø 15cm (≤2×106 cells/plate (15 mL/plate), 3x ≤6×106 cells/45 mL)

Materials & Methods

Materials

Method

6-wells
# 1 2 3
A
B
24-wells
# 1 2 3 4 5 6
A
B
C
D
96-wells plate
# 01 02 03 04 05 06 07 08 09 10 11 12
A
B
C
D
E
F
G
H
I

Results

  • WHAT?

Conclusion

  • DONE Ow..



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