User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/26: Difference between revisions
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===Preparation of Heat inactivated FBS/FCS=== | ===Preparation of Heat inactivated FBS/FCS=== | ||
* Defrost FBS @ 4 °C (over the weekend) | * Defrost FBS @ 4 °C (over the weekend) | ||
* Incubate <u>exactly</u> @ 56 °C and put to ice | * Incubate <u>exactly</u> 30 min. @ 56 °C and put to ice | ||
* Prepare sterile aliquots | * Prepare sterile aliquots | ||
* Store @ -20 °C | |||
==Notes== | ==Notes== |
Revision as of 05:42, 26 March 2010
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Summary
Materials & MethodsPlating cells from -80 °C
Preparation of Heat inactivated FBS/FCS
NotesResults
Discussion
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