User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/04/16: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Split cells for ELISA and secondary antibody Immunoblot</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Summary== | ==Summary== | ||
* 24-wells (6x 750.000 cells per well)& 10cm dishes (8x 1000000 cells per plate) plated out | * 24-wells (6x 750.000 cells per well)& 10cm dishes (8x 1000000 cells per plate) plated out (D9 P24) | ||
** probably too little cells on 24-wells due to miscalculation | ** probably too little cells on 24-wells due to miscalculation | ||
* Treated membrane with secondary antibody and obtained | * Treated membrane with secondary antibody and obtained | ||
** a lot of unspecicific binding on membrane | ** a lot of unspecicific binding on membrane | ||
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==Materials & Methods== | ==Materials & Methods== | ||
===Materials=== | ===Materials=== | ||
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</html> [[Media:FILE.xls| Excel TITLE]] | </html> [[Media:FILE.xls| Excel TITLE]]--> | ||
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Revision as of 01:27, 11 June 2010
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 Split cells for ELISA and secondary antibody Immunoblot
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Summary
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