User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/04/16

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Split cells for ELISA and secondary antibody Immunoblot Main project page
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Summary

  • 24-wells (6x 750.000 cells per well)& 10cm dishes (8x 1000000 cells per plate) plated out (D9 P24)
    • probably too little cells on 24-wells due to miscalculation
  • Treated membrane with secondary antibody and obtained
    • a lot of unspecicific binding on membrane


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