User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/04/21: Difference between revisions
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==Summary== | ==Summary== | ||
* cAMP precipitation | * cAMP precipitation (D9 P24) | ||
** including negative control, 19.5 mg of cAMP (BioLog) per mL lysate (1.8 mL was used) | ** including negative control, 19.5 mg of cAMP (BioLog) per mL lysate (1.8 mL was used) | ||
*** incubation was well over 30 minutes while shaking at 4 °C | *** incubation was well over 30 minutes while shaking at 4 °C | ||
** Samples are incubated with 8-HA-cAMP coated beads ON at 4 °C | ** Samples are incubated with 8-HA-cAMP coated beads ON at 4 °C | ||
* 24-wells stimulation | * 24-wells stimulation (D9 P24) | ||
** Not certain if concentrations are the same compared to Groningen | ** Not certain if concentrations are the same compared to Groningen | ||
*** 50 mM db-cAMP was diluted, 20 μL in 10 mL H<sub>2</sub>O | *** 50 mM db-cAMP was diluted, 20 μL in 10 mL H<sub>2</sub>O | ||
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* Stimulation used DMEM with 4.5 g/L | * Stimulation used DMEM with 4.5 g/L | ||
==Materials & Methods== | ==Materials & Methods== | ||
===Materials=== | ===Materials=== |
Revision as of 00:49, 28 April 2010
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Summary
Materials & MethodsMaterialsMethod
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