User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/04/21: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">cAMP precipitation, stimulation for ELISA</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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*** cBIMPs, MBC & 8Pcpt all 5 mM were diluted 200 μL in 10 mL H<sub>2</sub>O | *** cBIMPs, MBC & 8Pcpt all 5 mM were diluted 200 μL in 10 mL H<sub>2</sub>O | ||
*** Ht31 10 mM was used as provided | *** Ht31 10 mM was used as provided | ||
** All where added as described [[ | ** All where added as described [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/16| 16Feb2010]] with the cBIMPs, MBC and db-cAMP stocks being prepared as was 6-Bnz. However both cBIMPS and MBC where put into one vial, 300 μL each per 2 mL medium. | ||
*** First 20 min. incubation was extended to ~1 h due to circumstances | *** First 20 min. incubation was extended to ~1 h due to circumstances | ||
*** Second 25 min. incubation was also extended the same | *** Second 25 min. incubation was also extended the same | ||
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* Stimulation used DMEM with 4.5 g/L | * Stimulation used DMEM with 4.5 g/L | ||
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==Materials & Methods== | ==Materials & Methods== | ||
===Materials=== | ===Materials=== | ||
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Revision as of 01:29, 11 June 2010
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cAMP precipitation, stimulation for ELISA | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
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