User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/04/29: Difference between revisions
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(Autocreate 2010/04/29 Entry for User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization) |
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">SDS-PAGE& Finalizing RII overlay</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Summary== | ==Summary== | ||
* | * Picture RII overlay overnight | ||
* 2x 8% SD-PAGE Gel | |||
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| align="center" style="background:#f0f0f0;"|'''Lane''' | |||
| align="center" style="background:#f0f0f0;"|'''1''' | |||
| align="center" style="background:#f0f0f0;"|'''2''' | |||
| align="center" style="background:#f0f0f0;"|'''3''' | |||
| align="center" style="background:#f0f0f0;"|'''4''' | |||
| align="center" style="background:#f0f0f0;"|'''5''' | |||
| align="center" style="background:#f0f0f0;"|'''6''' | |||
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| align="center" style="background:#f0f0f0;"|'''8''' | |||
| align="center" style="background:#f0f0f0;"|'''9''' | |||
| align="center" style="background:#f0f0f0;"|'''10''' | |||
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| Gel #1||<center>Marker</center>||<center>S<sup>0</sup><br> Lysate (-)</center>||<center>S<sup>0</sup><br> NAC (-)</center>||<center>S<sup>0</sup><br> Beads Sample (-)</center>||<center>x</center>||<center>S<sup>0</sup><br> Lysate</center>||<center>S<sup>0</sup><br> NAC</center>||<center>S<sup>0</sup><br> Beads Sample</center>||<center>x</center>||<center>x</center> | |||
|- | |||
| Gel #2||<center>x<br> (some marker)</center>||<center>Marker</center>||<center>CSE<br> Lysate (-)</center>||<center>CSE<br> NAC (-)</center>||<center>CSE<br> Beads Sample (-)</center>||<center>x</center>||<center>CSE<br> Lysate</center>||<center>CSE<br> NAC </center>||<center>CSE<br> Beads Sample</center>||<center>x</center> | |||
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∞ NAC is supernatant left after incubation with beads | |||
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==Materials & Methods== | ==Materials & Methods== | ||
===Materials=== | ===Materials=== | ||
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</html> [[Media:FILE.xls| Excel TITLE]] | </html> [[Media:FILE.xls| Excel TITLE]]--> | ||
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__NOTOC__ | __NOTOC__ | ||
Revision as of 08:34, 26 May 2010
<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>
 SDS-PAGE& Finalizing RII overlay
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||
Summary
∞ NAC is supernatant left after incubation with beads | ||||||||||||||||||||||||||||||||||
