User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/04/30: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Splitting cells, Immunoblot</span> | ||
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* Antibody incubation for blot blocked ON | * Antibody incubation for blot blocked ON | ||
** mouse anti-RIIα (1:5000) for 2 hours @ RT | ** mouse anti-RIIα (1:5000) for 2 hours @ RT | ||
** Wash 3x 10 min. | ** Wash 3x 10 min. block buffer (1% BSA) | ||
** anti-mouse secondary antibody (1:10 000) 1 h @ RT | ** anti-mouse secondary antibody (1:10 000) 1 h @ RT | ||
** Rinse with block buffer | |||
** Wash 3x 15 min. w. block buffer | |||
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==Materials & Methods== | ==Materials & Methods== | ||
===Materials=== | ===Materials=== | ||
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Revision as of 08:34, 26 May 2010
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 Splitting cells, Immunoblot
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Summary
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