User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/05/06: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 14: Line 14:
** 6 mL CSE 100% in 40 mL gives 15%
** 6 mL CSE 100% in 40 mL gives 15%
** S<sup>0</sup> was not enough and due to the planned change of medium 50 mL of DMEM w/o antibiotics was added to the remaining DMEM S<sup>0</sup> (<10 mL) for the last two plates (marked with /)
** S<sup>0</sup> was not enough and due to the planned change of medium 50 mL of DMEM w/o antibiotics was added to the remaining DMEM S<sup>0</sup> (<10 mL) for the last two plates (marked with /)
*1xHBSS
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''MW (g/mol)'''
| align="center" style="background:#f0f0f0;"|'''Concentration (mM)'''
| align="center" style="background:#f0f0f0;"|'''Gram / Liter'''
|-
| Calcium Chloride dihydrate( CaCl2)||147.02||1,26||0.19
|-
| Potassium Chloride (KCL)||74.55||5,37||0.40
|-
| Potassium Phosphate Monobasic (KH2PO4)||136.09||0.44||0.06
|-
| Magnesium Chloride (MgCl2-6H2O)||203.31||0,49||0.10
|-
| Magnesium Sulphate anhydrous (MgSO4)||120.37||0,41||0.05
|-
| Sodium Chloride (NaCl)||58.44||136,89||8.00
|-
| Sodium Bicarbonate (NaHCO3)||84.01||4,17||0.35
|-
| Sodium Phosphate dihydrate (Na2HPO4)||156.01||0,34||0.05
|-
| Glucose (Dextrose)||180.16||5,55||1.00
|}


==Materials & Methods==
==Materials & Methods==

Revision as of 05:47, 6 May 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • 2x8 10cm dishes stimulated (15%CSE/S0) (10.45)
    • 6 mL CSE 100% in 40 mL gives 15%
    • S0 was not enough and due to the planned change of medium 50 mL of DMEM w/o antibiotics was added to the remaining DMEM S0 (<10 mL) for the last two plates (marked with /)
  • 1xHBSS
' MW (g/mol) Concentration (mM) Gram / Liter
Calcium Chloride dihydrate( CaCl2) 147.02 1,26 0.19
Potassium Chloride (KCL) 74.55 5,37 0.40
Potassium Phosphate Monobasic (KH2PO4) 136.09 0.44 0.06
Magnesium Chloride (MgCl2-6H2O) 203.31 0,49 0.10
Magnesium Sulphate anhydrous (MgSO4) 120.37 0,41 0.05
Sodium Chloride (NaCl) 58.44 136,89 8.00
Sodium Bicarbonate (NaHCO3) 84.01 4,17 0.35
Sodium Phosphate dihydrate (Na2HPO4) 156.01 0,34 0.05
Glucose (Dextrose) 180.16 5,55 1.00

Materials & Methods

Materials

Method

6-wells
# 1 2 3
A
B
24-wells
# 1 2 3 4 5 6
A
B
C
D
96-wells plate
# 01 02 03 04 05 06 07 08 09 10 11 12
A
B
C
D
E
F
G
H
I

Notes

Results

  • WHAT?

Discussion

  • DONE Ow..

Related

Related entries

Same actions

Related topics

Attachment

<html> <a href="http://openwetware.org/wiki/Image:HTERT_Donor_12_ht31_160310.xls"> 

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> Excel TITLE


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/06&oldid=413053"