User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/05/06: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Stimulation for RII overlay & Preparation of HBBS buffer for Alamar blue</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Summary== | ==Summary== | ||
* 6 mL CSE 100% in 40 mL gives 15% | *2x8 10cm dishes stimulated (15%CSE/S<sup>0</sup>) (10.45) | ||
** 6 mL CSE 100% in 40 mL gives 15% | |||
** S<sup>0</sup> was not enough and due to the planned change of medium 50 mL of DMEM w/o antibiotics was added to the remaining DMEM S<sup>0</sup> (<10 mL) for the last two plates (marked with /) | |||
*1xHBSS | |||
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* | | align="center" style="background:#f0f0f0;"|'''''' | ||
| align="center" style="background:#f0f0f0;"|'''MW (g/mol)''' | |||
== | | align="center" style="background:#f0f0f0;"|'''Concentration (mM)''' | ||
| align="center" style="background:#f0f0f0;"|'''Gram / Liter''' | |||
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| Calcium Chloride dihydrate( CaCl2)||147.02||1,26||0.19 | |||
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| | | Potassium Chloride (KCL)||74.55||5,37||0.40 | ||
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| Potassium Phosphate Monobasic (KH2PO4)||136.09||0.44||0.06 | |||
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| Magnesium Chloride (MgCl2-6H2O)||203.31||0,49||0.10 | |||
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| Magnesium Sulphate anhydrous (MgSO4)||120.37||0,41||0.05 | |||
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| Sodium Chloride (NaCl)||58.44||136,89||8.00 | |||
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| | | Sodium Bicarbonate (NaHCO3)||84.01||4,17||0.35 | ||
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| Sodium Phosphate dihydrate (Na2HPO4)||156.01||0,34||0.05 | |||
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| Glucose (Dextrose)||180.16||5,55||1.00 | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 08:32, 26 May 2010
<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>
Stimulation for RII overlay & Preparation of HBBS buffer for Alamar blue | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||
Summary
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