User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/05/06: Difference between revisions

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{|{{table}} width="800"
{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Stimulation for RII overlay & Preparation of HBBS buffer for Alamar blue</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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** S<sup>0</sup> was not enough and due to the planned change of medium 50 mL of DMEM w/o antibiotics was added to the remaining DMEM S<sup>0</sup> (<10 mL) for the last two plates (marked with /)
** S<sup>0</sup> was not enough and due to the planned change of medium 50 mL of DMEM w/o antibiotics was added to the remaining DMEM S<sup>0</sup> (<10 mL) for the last two plates (marked with /)


==Materials & Methods==
*1xHBSS
===Materials===
{| {{table}}
*  
| align="center" style="background:#f0f0f0;"|''''''
 
| align="center" style="background:#f0f0f0;"|'''MW (g/mol)'''
===Method===
| align="center" style="background:#f0f0f0;"|'''Concentration (mM)'''
{| border="1"
| align="center" style="background:#f0f0f0;"|'''Gram / Liter'''
|+'''6-wells'''
!#
!<u>1</u>
!<u>2</u>
!<u>3</u>
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!A
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!B
| Calcium Chloride dihydrate( CaCl2)||147.02||1,26||0.19
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| Potassium Chloride (KCL)||74.55||5,37||0.40
{| border="1"
|+'''24-wells'''
!#
!<u>1</u>
!<u>2</u>
!<u>3</u>
!<u>4</u>
!<u>5</u>
!<u>6</u>
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!A
| Potassium Phosphate Monobasic (KH2PO4)||136.09||0.44||0.06
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!B
| Magnesium Chloride (MgCl2-6H2O)||203.31||0,49||0.10
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!C
| Magnesium Sulphate anhydrous (MgSO4)||120.37||0,41||0.05
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!D
| Sodium Chloride (NaCl)||58.44||136,89||8.00
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| Sodium Bicarbonate (NaHCO3)||84.01||4,17||0.35
{| border="1"
|+'''96-wells plate'''
|#
!01
!02
!03
!04
!05
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!12
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!A
| Sodium Phosphate dihydrate (Na2HPO4)||156.01||0,34||0.05
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| Glucose (Dextrose)||180.16||5,55||1.00
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==Notes==
==Results==
*{{todo| WHAT?}}
==Discussion==
*{{done}} Ow..


==Related==
===Related entries===
===Same actions===
===Related topics===
==Attachment==
<html>
<a href="http://openwetware.org/wiki/Image:HTERT_Donor_12_ht31_160310.xls">
  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
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</html> [[Media:FILE.xls| Excel TITLE]]
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__NOTOC__
__NOTOC__

Revision as of 08:32, 26 May 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Stimulation for RII overlay & Preparation of HBBS buffer for Alamar blue <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • 2x8 10cm dishes stimulated (15%CSE/S0) (10.45)
    • 6 mL CSE 100% in 40 mL gives 15%
    • S0 was not enough and due to the planned change of medium 50 mL of DMEM w/o antibiotics was added to the remaining DMEM S0 (<10 mL) for the last two plates (marked with /)
  • 1xHBSS
' MW (g/mol) Concentration (mM) Gram / Liter
Calcium Chloride dihydrate( CaCl2) 147.02 1,26 0.19
Potassium Chloride (KCL) 74.55 5,37 0.40
Potassium Phosphate Monobasic (KH2PO4) 136.09 0.44 0.06
Magnesium Chloride (MgCl2-6H2O) 203.31 0,49 0.10
Magnesium Sulphate anhydrous (MgSO4) 120.37 0,41 0.05
Sodium Chloride (NaCl) 58.44 136,89 8.00
Sodium Bicarbonate (NaHCO3) 84.01 4,17 0.35
Sodium Phosphate dihydrate (Na2HPO4) 156.01 0,34 0.05
Glucose (Dextrose) 180.16 5,55 1.00



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