|
|
Line 41: |
Line 41: |
| |} | | |} |
|
| |
|
| ==Materials & Methods==
| |
| ===Materials===
| |
| *
| |
|
| |
|
| ===Method===
| |
| {| border="1"
| |
| |+'''6-wells'''
| |
| !#
| |
| !<u>1</u>
| |
| !<u>2</u>
| |
| !<u>3</u>
| |
| |-
| |
| !A
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| !B
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| |}
| |
| {| border="1"
| |
| |+'''24-wells'''
| |
| !#
| |
| !<u>1</u>
| |
| !<u>2</u>
| |
| !<u>3</u>
| |
| !<u>4</u>
| |
| !<u>5</u>
| |
| !<u>6</u>
| |
| |-
| |
| !A
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| !B
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| !C
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| !D
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| |}
| |
| {| border="1"
| |
| |+'''96-wells plate'''
| |
| |#
| |
| !01
| |
| !02
| |
| !03
| |
| !04
| |
| !05
| |
| !06
| |
| !07
| |
| !08
| |
| !09
| |
| !10
| |
| !11
| |
| !12
| |
| |-
| |
| !A
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| !B
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| !C
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| !D
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| !E
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| !F
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| !G
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| !H
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| !I
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |-
| |
| |}
| |
|
| |
| ==Notes==
| |
| ==Results==
| |
| *{{todo| WHAT?}}
| |
|
| |
| ==Discussion==
| |
| *{{done}} Ow..
| |
|
| |
| ==Related==
| |
| ===Related entries===
| |
| ===Same actions===
| |
| ===Related topics===
| |
| ==Attachment==
| |
| <html>
| |
| <a href="http://openwetware.org/wiki/Image:HTERT_Donor_12_ht31_160310.xls">
| |
| <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
| |
| </a>
| |
| </html> [[Media:FILE.xls| Excel TITLE]]
| |
| <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
| |} | | |} |
|
| |
|
| __NOTOC__ | | __NOTOC__ |
Revision as of 08:32, 26 May 2010
<html><style type="text/css">
.todo { color: red }
.done { color: green}
</style></html>
Stimulation for RII overlay & Preparation of HBBS buffer for Alamar blue
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
|
Summary
- 2x8 10cm dishes stimulated (15%CSE/S0) (10.45)
- 6 mL CSE 100% in 40 mL gives 15%
- S0 was not enough and due to the planned change of medium 50 mL of DMEM w/o antibiotics was added to the remaining DMEM S0 (<10 mL) for the last two plates (marked with /)
'
|
MW (g/mol)
|
Concentration (mM)
|
Gram / Liter
|
Calcium Chloride dihydrate( CaCl2) |
147.02 |
1,26 |
0.19
|
Potassium Chloride (KCL) |
74.55 |
5,37 |
0.40
|
Potassium Phosphate Monobasic (KH2PO4) |
136.09 |
0.44 |
0.06
|
Magnesium Chloride (MgCl2-6H2O) |
203.31 |
0,49 |
0.10
|
Magnesium Sulphate anhydrous (MgSO4) |
120.37 |
0,41 |
0.05
|
Sodium Chloride (NaCl) |
58.44 |
136,89 |
8.00
|
Sodium Bicarbonate (NaHCO3) |
84.01 |
4,17 |
0.35
|
Sodium Phosphate dihydrate (Na2HPO4) |
156.01 |
0,34 |
0.05
|
Glucose (Dextrose) |
180.16 |
5,55 |
1.00
|
|