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 Stimulation for RII overlay & Preparation of HBBS buffer for Alamar blue
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Summary
- 2x8 10cm dishes stimulated (15%CSE/S0) (10.45)
- 6 mL CSE 100% in 40 mL gives 15%
- S0 was not enough and due to the planned change of medium 50 mL of DMEM w/o antibiotics was added to the remaining DMEM S0 (<10 mL) for the last two plates (marked with /)
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MW (g/mol)
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Concentration (mM)
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Gram / Liter
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| Calcium Chloride dihydrate( CaCl2) |
147.02 |
1,26 |
0.19
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| Potassium Chloride (KCL) |
74.55 |
5,37 |
0.40
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| Potassium Phosphate Monobasic (KH2PO4) |
136.09 |
0.44 |
0.06
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| Magnesium Chloride (MgCl2-6H2O) |
203.31 |
0,49 |
0.10
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| Magnesium Sulphate anhydrous (MgSO4) |
120.37 |
0,41 |
0.05
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| Sodium Chloride (NaCl) |
58.44 |
136,89 |
8.00
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| Sodium Bicarbonate (NaHCO3) |
84.01 |
4,17 |
0.35
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| Sodium Phosphate dihydrate (Na2HPO4) |
156.01 |
0,34 |
0.05
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| Glucose (Dextrose) |
180.16 |
5,55 |
1.00
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Stimulation for RII overlay & Preparation of HBBS buffer for Alamar blue
