User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/05/06

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Stimulation for RII overlay & Preparation of HBBS buffer for Alamar blue Main project page
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  • 2x8 10cm dishes stimulated (15%CSE/S0) (10.45)
    • 6 mL CSE 100% in 40 mL gives 15%
    • S0 was not enough and due to the planned change of medium 50 mL of DMEM w/o antibiotics was added to the remaining DMEM S0 (<10 mL) for the last two plates (marked with /)
  • 1xHBSS
' MW (g/mol) Concentration (mM) Gram / Liter
Calcium Chloride dihydrate( CaCl2)147.021,260.19
Potassium Chloride (KCL)74.555,370.40
Potassium Phosphate Monobasic (KH2PO4)136.090.440.06
Magnesium Chloride (MgCl2-6H2O)203.310,490.10
Magnesium Sulphate anhydrous (MgSO4)120.370,410.05
Sodium Chloride (NaCl)58.44136,898.00
Sodium Bicarbonate (NaHCO3)84.014,170.35
Sodium Phosphate dihydrate (Na2HPO4)156.010,340.05
Glucose (Dextrose)180.165,551.00

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