User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/05/09: Difference between revisions

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==Discussion==
==Discussion==
*{{done}} Ow..
* A band around 55 kDa is shown only concentrated in the cAMP pull down
** This would be RII subunits showing that cAMP precipitation is succesful
* cAMP treated beads pull down don't show any bands, indicating that cAMP prevented the binding of samples to cAMP bound to the beads


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Revision as of 08:30, 26 May 2010

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SDS-PAGE of cAMP precipitation <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • Short summary

Materials & Methods

Method

  • Put samples to 8% polyacrylamide gell
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Sample Marker CSE beads CSE NAC CSE Lysate CSE (-) beads CSE (-) NAC CSE (-) Lysate x S0 beads S0 NAC S0 Lysate S0 (-) beads S0 (-) NAC S0 (-) Lysate marker

Notes

  • Slot 8 was filled and slot 15 had no marker added

Results

  • Ponceau staining of cAMP precipitation on 8% Gel

Discussion

  • A band around 55 kDa is shown only concentrated in the cAMP pull down
    • This would be RII subunits showing that cAMP precipitation is succesful
  • cAMP treated beads pull down don't show any bands, indicating that cAMP prevented the binding of samples to cAMP bound to the beads


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