User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/05/09: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">SDS-PAGE of cAMP precipitation</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Summary==
 
* Short summary


==Materials & Methods==
==Materials & Methods==
===Materials===
 
*


===Method===
===Method===
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* Put samples to 8% polyacrylamide gell
|+'''6-wells'''
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| Sample||Marker||CSE beads||CSE NAC||CSE Lysate||CSE (-) beads||CSE (-) NAC||CSE (-) Lysate||x||S<sup>0</sup> beads||S<sup>0</sup> NAC||S<sup>0</sup> Lysate||S<sup>0</sup> (-) beads||S<sup>0</sup> (-) NAC||S<sup>0</sup> (-) Lysate||marker
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==Notes==
==Notes==
*Slot 8 was filled and slot 15 had no marker added
==Results==
==Results==
*{{todo| WHAT?}}
*Ponceau staining of cAMP precipitation on 8% Gel
 
[[Image:100509_ponceau_cAMP_%26_lysates.tif|500px]]


==Discussion==
==Discussion==
*{{done}} Ow..
* A band around 55 kDa is shown only concentrated in the cAMP pull down
** This would be RII subunits showing that cAMP precipitation is succesful
* cAMP treated beads pull down don't show any bands, indicating that cAMP prevented the binding of samples to cAMP bound to the beads


==Related==
===Related entries===
===Same actions===
===Related topics===
==Attachment==
<html>
<a href="http://openwetware.org/wiki/Image:HTERT_Donor_12_ht31_160310.xls">
  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
</a>
</html> [[Media:FILE.xls| Excel TITLE]]
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__NOTOC__
__NOTOC__

Revision as of 08:30, 26 May 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

SDS-PAGE of cAMP precipitation <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Materials & Methods

Method

  • Put samples to 8% polyacrylamide gell
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Sample Marker CSE beads CSE NAC CSE Lysate CSE (-) beads CSE (-) NAC CSE (-) Lysate x S0 beads S0 NAC S0 Lysate S0 (-) beads S0 (-) NAC S0 (-) Lysate marker

Notes

  • Slot 8 was filled and slot 15 had no marker added

Results

  • Ponceau staining of cAMP precipitation on 8% Gel

Discussion

  • A band around 55 kDa is shown only concentrated in the cAMP pull down
    • This would be RII subunits showing that cAMP precipitation is succesful
  • cAMP treated beads pull down don't show any bands, indicating that cAMP prevented the binding of samples to cAMP bound to the beads


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