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 SDS-PAGE of cAMP precipitation
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Materials & Methods
Method
- Put samples to 8% polyacrylamide gell
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1
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2
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3
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4
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5
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6
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7
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8
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9
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10
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11
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12
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13
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14
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15
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| Sample |
Marker |
CSE beads |
CSE NAC |
CSE Lysate |
CSE (-) beads |
CSE (-) NAC |
CSE (-) Lysate |
x |
S0 beads |
S0 NAC |
S0 Lysate |
S0 (-) beads |
S0 (-) NAC |
S0 (-) Lysate |
marker
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Notes
- Slot 8 was filled and slot 15 had no marker added
Results
- Ponceau staining of cAMP precipitation on 8% Gel
Discussion
- A band around 55 kDa is shown only concentrated in the cAMP pull down
- This would be RII subunits showing that cAMP precipitation is succesful
- cAMP treated beads pull down don't show any bands, indicating that cAMP prevented the binding of samples to cAMP bound to the beads
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SDS-PAGE of cAMP precipitation
