User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/05/31

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<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

SDS-PAGE for AKAP79 immunoblot & RII overlay (HEK293) <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

SDS-PAGE

Biorad Precision Plus Protein Dual Color marker
Biorad Precision Plus Protein Dual Color marker
#1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Sample Marker hTERT RIPA hTERT Potter HEK293 RIPA Marker hTERT RIPA hTERT Potter HEK293 RIPA x x x x x x x
#2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Sample Marker hTERT RIPA hTERT Potter HEK293 RIPA Marker hTERT RIPA hTERT Potter HEK293 RIPA x x x x x x x

siRNA prep

  • Splitting cells into 12-wells?


Notes

  • Wells of gel #1 were not great in that they appeared not to be perfectly separated at the bottom

Results

  • WHAT?

Discussion

  • DONE Ow..


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