User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/01: Difference between revisions

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===Method===
===Method===
{| border="1"
{| {{table}}
|+'''6-wells'''
| align="center" style="background:#f0f0f0;"|'''#2'''
!#
| align="center" style="background:#f0f0f0;"|'''1'''
!<u>1</u>
| align="center" style="background:#f0f0f0;"|'''2'''
!<u>2</u>
| align="center" style="background:#f0f0f0;"|'''3'''
!<u>3</u>
| align="center" style="background:#f0f0f0;"|'''4'''
| align="center" style="background:#f0f0f0;"|'''5'''
| align="center" style="background:#f0f0f0;"|'''6'''
| align="center" style="background:#f0f0f0;"|'''7'''
| align="center" style="background:#f0f0f0;"|'''8'''
| align="center" style="background:#f0f0f0;"|'''9'''
| align="center" style="background:#f0f0f0;"|'''10'''
| align="center" style="background:#f0f0f0;"|'''11'''
| align="center" style="background:#f0f0f0;"|'''12'''
| align="center" style="background:#f0f0f0;"|'''13'''
| align="center" style="background:#f0f0f0;"|'''14'''
| align="center" style="background:#f0f0f0;"|'''15'''
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!A
| Sample||[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/08|hTERT RIPA]]||[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/07|hTERT Potter]]||[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/27|HEK293 RIPA]]||Marker||[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/08|hTERT RIPA]]||[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/07|hTERT Potter]]||[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/27|HEK293 RIPA]]||Marker||[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/08|hTERT RIPA]]||[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/07|hTERT Potter]]||[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/27|HEK293 RIPA]]||Marker||[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/08|hTERT RIPA]]||[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/07|hTERT Potter]]||[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/27|HEK293 RIPA]]
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{| border="1"
|+'''24-wells'''
!#
!<u>1</u>
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|+'''96-wells plate'''
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Revision as of 06:24, 1 June 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • Gel #2 was tried with fresh APS 10% (previous was 1 month old)
    • Still large blur was seen during and after running the gel.
    • When checking pH of the stacking gel a pH of 7.15 was found instead of 6.8
    • This will prevent the stacking function and would explain the blur seen while running
  • New stacking buffer was prepared
    • 18.9 g TRIS
    • 150 mL dH2O
    • pH 6.8
    • added to 250 mL with dH2O
  • Gel #3 was prepared
    • All other gels stored at 4 °C were discarded

Materials & Methods

Materials

Method

#2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Sample hTERT RIPA hTERT Potter HEK293 RIPA Marker hTERT RIPA hTERT Potter HEK293 RIPA Marker hTERT RIPA hTERT Potter HEK293 RIPA Marker hTERT RIPA hTERT Potter HEK293 RIPA

Notes

Results

  • WHAT?

Discussion

  • DONE Ow..

Related

Related entries

Same actions

Related topics

Attachment

<html> <a href="http://openwetware.org/wiki/Image:HTERT_Donor_12_ht31_160310.xls"> 

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> Excel TITLE


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