User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/10: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Put cells to S<sup>0</sup>, split cells</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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* Split D9 cells to P28 (18 new plates)
* Split D9 cells to P28 (18 new plates)
* Checked Smoking machine and another malfunction has occured. Probably rust or it has trouble with the hot weather
* Checked Smoking machine and another malfunction has occured. Probably rust or it has trouble with the hot weather
 
* While writing paper I found that the anti-AKAP12 antibody handed to me on [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/15|15April2010]] was not against the human, but Rat AKAP12.
** In future Santa cruz antibody which is also present should be used
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Revision as of 00:48, 11 June 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Put cells to S0, split cells <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • Put cells D9 P27 to S0 (14 plates)
  • Split D9 cells to P28 (18 new plates)
  • Checked Smoking machine and another malfunction has occured. Probably rust or it has trouble with the hot weather
  • While writing paper I found that the anti-AKAP12 antibody handed to me on 15April2010 was not against the human, but Rat AKAP12.
    • In future Santa cruz antibody which is also present should be used