User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/13: Difference between revisions
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* Protein pellets left @ 4 °C have been resuspended while in storage, only minor pellet remains | * Protein pellets left @ 4 °C have been resuspended while in storage, only minor pellet remains | ||
** For final resuspending samples were incubated @ 50 °C and vortexed until resuspended | ** For final resuspending samples were incubated @ 50 °C and vortexed until resuspended | ||
* Cells of D9 P28 were put to S<sup>0</sup> | |||
* Cells were split to D9 P29 (800.000 cells per plate) | |||
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*Cells were letting go a bit during washing, perhaps too confluent and dying? | |||
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Revision as of 01:14, 17 June 2010
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 Putting cells to S0, splitting cells and continuing of protein isolation
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