User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/13

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Putting cells to S0, splitting cells and continuing of protein isolation <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • Protein pellets left @ 4 °C have been resuspended while in storage, only minor pellet remains
    • For final resuspending samples were incubated @ 50 °C and vortexed until resuspended
  • Cells of D9 P28 were put to S0
  • Cells were split to D9 P29 (800.000 cells per plate)

Materials & Methods

Materials

Method

6-wells
# 1 2 3
A
B
24-wells
# 1 2 3 4 5 6
A
B
C
D
96-wells plate
# 01 02 03 04 05 06 07 08 09 10 11 12
A
B
C
D
E
F
G
H
I

Notes

Results

  • WHAT?

Discussion

  • DONE Ow..

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