User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/15: Difference between revisions
From OpenWetWare
No edit summary |
|||
(4 intermediate revisions by the same user not shown) | |||
Line 5: | Line 5: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Stop stimulation: Protein and RNA/Protein isolation(2)</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Stop stimulation: Protein and RNA/Protein isolation (2)</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
|- | |- | ||
Line 14: | Line 14: | ||
* 12 plates (6 and 6) were lysed with RIPA as described [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/24| 24Feb2010]] for protein isolation | * 12 plates (6 and 6) were lysed with RIPA as described [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/24| 24Feb2010]] for protein isolation | ||
* 2 platse (1 and 1) were lysed as described [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/08| 8June2010]] for RNA (and protein) isolation | * 2 platse (1 and 1) were lysed as described [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/08| 8June2010]] for RNA (and protein) isolation | ||
** RNA concentrations, see attachment | |||
** For protein isolation from the phenol/chloroform fraction the pellet obtained was mechanically disrupted using a pipette point | |||
* AT 22.00 ~ 22.30 cells of D9 P29 were put to S<sup>0</sup>, cells were less confluent (~90%) but this is believed to better then previous where cells let go of surface during washing and appeared to be dying. | |||
<!-- | |||
==Materials & Methods== | ==Materials & Methods== | ||
===Materials=== | ===Materials=== | ||
Line 236: | Line 239: | ||
===Same actions=== | ===Same actions=== | ||
===Related topics=== | ===Related topics=== | ||
--> | |||
==Attachment== | ==Attachment== | ||
<html> | <html> | ||
<a href="http://openwetware.org/wiki/Image: | <a href="http://openwetware.org/wiki/Image:15062010_NanodropRNAconcentration.pdf"> | ||
<img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> | <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> | ||
</a> | </a> | ||
</html> [[Media: | </html> [[Media:15062010_NanodropRNAconcentration.pdf|Nanodrop of RNA concentrations]] | ||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
|} | |} | ||
__NOTOC__ | __NOTOC__ |
Revision as of 01:06, 23 June 2010
<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>
Stop stimulation: Protein and RNA/Protein isolation (2) | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Summary
Attachment<html> <a href="http://openwetware.org/wiki/Image:15062010_NanodropRNAconcentration.pdf"> <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> </a> </html> Nanodrop of RNA concentrations |