User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/15: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Stop stimulation: Protein and RNA/Protein isolation(2)</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Stop stimulation: Protein and RNA/Protein isolation (2)</span> | ||
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** RNA concentrations, see attachment | ** RNA concentrations, see attachment | ||
** For protein isolation from the phenol/chloroform fraction the pellet obtained was mechanically disrupted using a pipette point | ** For protein isolation from the phenol/chloroform fraction the pellet obtained was mechanically disrupted using a pipette point | ||
* AT 22.00 ~ 22.30 cells of D9 P29 were put to S<sup>0</sup>, cells were less confluent (~90%) but this is believed to better then previous where cells let go of surface during washing and appeared to be dying. | |||
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</html> [[Media: | </html> [[Media:15062010_NanodropRNAconcentration.pdf|Nanodrop of RNA concentrations]] | ||
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Revision as of 01:06, 23 June 2010
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 Stop stimulation: Protein and RNA/Protein isolation (2)
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Summary
Attachment<html> <a href="http://openwetware.org/wiki/Image:15062010_NanodropRNAconcentration.pdf"> <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> </a> </html> Nanodrop of RNA concentrations | |
