User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/17: Difference between revisions

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* Protein isolation after RNA isolation was performed ONLY until pellet was obtained
* Protein isolation after RNA isolation was performed ONLY until pellet was obtained
** Pellet was stored at -20 °C
** Pellet was stored at -20 °C
 
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Revision as of 13:17, 19 August 2010

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Stop stimulation: Protein and RNA/Protein isolation(3) <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • All 20 plates of D9 P29 were washed twice with cold PBS
  • 16 plates (8 and 8) were lysed with RIPA as described 24Feb2010 for protein isolation
  • 4 plates (2 and 2) were lysed as described 8June2010 for RNA (and protein) isolation

Notes

  • Protein isolation after RNA isolation was performed ONLY until pellet was obtained
    • Pellet was stored at -20 °C

Attachment

<html> <a href="http://openwetware.org/wiki/Image:17062010_NanodropRNAconcentration.pdf"> 

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> Nanodrop concentrations


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