User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/17

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Stop stimulation: Protein and RNA/Protein isolation(3) Main project page
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Summary

  • All 20 plates of D9 P29 were washed twice with cold PBS
  • 16 plates (8 and 8) were lysed with RIPA as described 24Feb2010 for protein isolation
  • 4 plates (2 and 2) were lysed as described 8June2010 for RNA (and protein) isolation

Notes

  • Protein isolation after RNA isolation was performed ONLY until pellet was obtained
    • Pellet was stored at -20 °C

Attachment

Nanodrop concentrations


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