User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/21: Difference between revisions

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==Summary==
==Summary==
* Short summary
*12June2010: CSE & S<sup>0</sup> (hTERT D9 P27)
*15June2010: CSE & S<sup>0</sup> (hTERT D9 P28)
*17June2010: CSE & S<sup>0</sup> (hTERT D9 P29)
 
===Bradford assay===
*Dilute samples 5x (4 μL samples + 16 μL H2O)
*Put (3x) 5 μL sample on plate
*Put STND on plate
*Add 250 μL Bradford reagent
*Measure (AG KLUSSMANN FRANK BRADFORD ASSAY)


==Materials & Methods==
==Materials & Methods==

Revision as of 02:09, 21 June 2010

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Summary

  • 12June2010: CSE & S0 (hTERT D9 P27)
  • 15June2010: CSE & S0 (hTERT D9 P28)
  • 17June2010: CSE & S0 (hTERT D9 P29)

Bradford assay

  • Dilute samples 5x (4 μL samples + 16 μL H2O)
  • Put (3x) 5 μL sample on plate
  • Put STND on plate
  • Add 250 μL Bradford reagent
  • Measure (AG KLUSSMANN FRANK BRADFORD ASSAY)

Materials & Methods

Materials

Method

6-wells
# 1 2 3
A
B
24-wells
# 1 2 3 4 5 6
A
B
C
D
96-wells plate
# 01 02 03 04 05 06 07 08 09 10 11 12
A
B
C
D
E
F
G
H
I

Notes

Results

  • WHAT?

Discussion

  • DONE Ow..

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