User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/21: Difference between revisions
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*15June2010: CSE & S<sup>0</sup> (hTERT D9 P28) | *15June2010: CSE & S<sup>0</sup> (hTERT D9 P28) | ||
*17June2010: CSE & S<sup>0</sup> (hTERT D9 P29) | *17June2010: CSE & S<sup>0</sup> (hTERT D9 P29) | ||
* Got some new HEK293 cells, about 7.5 mL (2·10<sup>6</sup> cells/mL) | |||
** Spinned down 1000 rcf 5 min. | |||
*** NOTE: First 4000 rcf for a few seconds! (maybe not nice for cells, but needed to lyse them anyway) | |||
** Added 2 mL of RIPA lysis buffer and sonicated them | |||
===Bradford assay=== | ===Bradford assay=== | ||
*Dilute samples 5x (4 μL samples + 16 μL H2O) | *Dilute samples 5x (4 μL samples + 16 μL H2O) | ||
Revision as of 02:31, 21 June 2010
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Summary
Bradford assay
Materials & MethodsMaterialsMethod
NotesResults
Discussion
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