User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/22: Difference between revisions
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*Gel running was troublesome, first a bit blurry but straightened out later. Marker was a bit faint and running took >2 h. | *Gel running was troublesome, first a bit blurry but straightened out later. Marker was a bit faint and running took >2 h. | ||
**HiMark (Invitrogen) was used | **HiMark (Invitrogen) was used | ||
* Upper 3 bands were not completely transferred to the membrane | |||
==Results== | ==Results== | ||
* | *Ponceau staining of cAMP precipitations 4 gels with the same samples and marker | ||
[[Image:22062010_Ponceau_staining_cAMP_precipitation.png|500px]] | |||
==Discussion== | ==Discussion== | ||
* | *Nothing visible, HOPEFULLY this is due to a low amount of protein (or bad Ponceau) and NOT due to the complete lack of protein | ||
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==Related== | ==Related== | ||
===Related entries=== | ===Related entries=== |
Revision as of 09:42, 22 June 2010
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SummaryWestern blot
Washing beads: cAMP precipitation
RIPA BUFFER (100 mL)
Notes
Results
Discussion
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