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 Immunoblot repeat
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Summary
- To check if really nothing could be detected the same antibodies were used ON, repeat of yesterday.
Notes
- The rotating device to turn gels ON had broken down, but were switched to different machine by Philipp
Results
Gel loading hTERT D9 + HEK293 see codes 21June2010
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1
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2
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3
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4
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5
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6
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7
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8
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9
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10
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Gel
cAMP prec.
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X
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HEK293
(20 μL)
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Marker
(8 μL)
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C1
(20 μL)
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C2
(20 μL)
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C3
(20 μL)
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S1
(20 μL)
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S2
(20 μL)
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S3
(20 μL)
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X
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Discussion
- RIIα detection went fine now, no explanation why it didn't work before like the rest
- The other antibodies still could not find anything
- To see if it is due to the transfer to the membrane a new gel will be run and stained (Coomassie or Silver)
- Another possibility is to do the RII overlay on the remaining blot of 22June2010 to see if there is anything that was precipitated together with RII subunits
- As a positive control for AKAP95 and AKAP450 HeLa cells have been requested
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Immunoblot repeat
