User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/25: Difference between revisions
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*It was mentioned that the Perkin-Elmer device used for the Bradford method for protein concentration is somehow defect and not giving proper measurements. This might have led to the wrong protein concentrations and a too high dilution of the samples. | *It was mentioned that the Perkin-Elmer device used for the Bradford method for protein concentration is somehow defect and not giving proper measurements. This might have led to the wrong protein concentrations and a too high dilution of the samples. | ||
*Protein concentration needs to be re-determined | *Protein concentration needs to be re-determined | ||
*Perhaps the bands seen ~116 kDa are Epac2, also due to the fact that they are downregulated after CSE induction | |||
==Related== | ==Related== |
Revision as of 07:47, 25 June 2010
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SummaryCoomassie staining
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