User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/25: Difference between revisions
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==Results== | ==Results== | ||
*The amount of protein in the samples apears to be very low, bands around the size of the RII subunits (50 ~ 55 kDa) can be barely seen. Also between 116 - 150 kDa a faint band is seen. | *The amount of protein in the samples apears to be very low, bands around the size of the RII subunits (50 ~ 55 kDa) can be barely seen. Also between 116 - 150 kDa a faint band is seen. | ||
*500 kDa band appears to reside in the stacking gel still, next time run gel further (although I usually don't make such large stacking gels) | |||
[[Image:25062010_cAMP_precipitation_Coomassie.png|500px]] | [[Image:25062010_cAMP_precipitation_Coomassie.png|500px]] | ||
*For better resolution gel will be destained over the weekend | |||
==Discussion== | ==Discussion== | ||
*It was mentioned that the Perkin-Elmer device used for the Bradford method for protein concentration is somehow defect and not giving proper measurements. This might have led to the wrong protein concentrations and a too high dilution of the samples. | *It was mentioned that the Perkin-Elmer device used for the Bradford method for protein concentration is somehow defect and not giving proper measurements. This might have led to the wrong protein concentrations and a too high dilution of the samples. | ||
*Protein concentration needs to be re-determined | *Protein concentration needs to be re-determined | ||
*Perhaps the bands seen ~116 kDa are Epac2, also due to the fact that they are downregulated after CSE induction | |||
==Related== | ==Related== |
Revision as of 08:25, 25 June 2010
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SummaryCoomassie staining
Notes
Results
Discussion
RelatedRelated topics |