User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/25

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Coomassie staining <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

COOMASSIE STAINING

  1. Run SDS-PAGE (8%)
  2. Fix in fixing solution (40% EtOH, 10% Acetic Acid) 10 min. @ RT
  3. Stain with staining solution () for 30 min. @ RT (Heat in Microwave to speed up process)
  4. Destain in destaining solution () for as long a necessary
    1. Put Absorbing material on top of gel to catch excess coomassie


Gel loading hTERT D9 + HEK293 see codes 21June2010
# 1 2 3 4 5 6 7 8 9 10
Gel
cAMP prec. 		Biorad Marker
(8 μL) 		HEK293
(20 μL) 		Invitrogen Marker
(8 μL) 		C1
(20 μL) 		C2
(20 μL) 		C3
(20 μL) 		S1
(20 μL) 		S2
(20 μL) 		S3
(20 μL) 		X


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