User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/28: Difference between revisions
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==Summary== | ==Summary== | ||
* | ===Protein work=== | ||
* HeLa cell lysis as described [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/04/08|08April2010]] | |||
* Protein determination as described [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] | |||
** HeLa | |||
** HEK293 | |||
** hTERT ASM Samples | |||
*cAMP precipitation | |||
* SDS-PAGE | |||
** RII Overlay | |||
** Silver / Coomassie staining | |||
** Immunoblot | |||
*** AKAP250 | |||
*** AKAP95 | |||
*** AKAP450 | |||
===RNA work== | |||
* Preparing cDNA | |||
* Real time qPCR | |||
==Materials & Methods== | ==Materials & Methods== |
Revision as of 01:24, 28 June 2010
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SummaryProtein work
=RNA work
Materials & MethodsMaterialsMethod
NotesResults
Discussion
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