User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/28: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">HeLa lysis, Bradford & cAMP precipitation part I</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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Revision as of 01:20, 29 June 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

HeLa lysis, Bradford & cAMP precipitation part I <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

Protein work

  • HeLa (Passage 16) cell lysis as described 08April2010
    • 500 μL RIPA buffer for T25 flask (2 more T75 flasks were ordered)
  • Protein determination as described 21June2010
    • HeLa
    • HEK293
    • hTERT ASM Samples
  • cAMP precipitation
    • Everything was put on beads corrected to the lowest concentration, to at least get similar relative amounts (see appendix)
    • Beads were incubate ON @ 4 °C

Attachment

<html> <a href="http://openwetware.org/wiki/Image:Bradford_28062010.xls"> 

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> Bradford results


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