User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/28

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HeLa lysis, Bradford & cAMP precipitation part I <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

Protein work

  • HeLa (Passage 16) cell lysis as described 08April2010
    • 500 μL RIPA buffer for T25 flask (2 more T75 flasks were ordered)
  • Protein determination as described 21June2010
    • HeLa
    • HEK293
    • hTERT ASM Samples
  • cAMP precipitation
    • Everything was put on beads corrected to the lowest concentration, to at least get similar relative amounts (see appendix)
    • Beads were incubate ON @ 4 °C

Attachment

<html> <a href="http://openwetware.org/wiki/Image:Bradford_28062010.xls"> 

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> Bradford results


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