User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/29: Difference between revisions
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* Preparing cDNA | * Preparing cDNA | ||
* Real time qPCR | * Real time qPCR | ||
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*cAMP precipitations had 50 μL of 4x loading buffer put on them, they should have been boiled for 5 - 10 min. but they were boiled approx. 2 hours | |||
** Hardly any blue is still seen in soluble fraction, probably samples are ruined | |||
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Revision as of 07:21, 29 June 2010
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 cAMP precipitation part II & SDS-PAGE
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SummaryWashing beads: cAMP precipitation
Protein work
RNA work
Notes
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