User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/01: Difference between revisions

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==Notes==
==Notes==
==Results==
==Results==
*{{todo| WHAT?}}
*Coomassie staining
[[Image:01072010_cAMP_precipitation_Coomassie.png|500px]]


==Discussion==
==Discussion==

Revision as of 02:27, 2 July 2010

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Summary

  • 8% SDS-PAGE, Coomassie staining and silver staining
  • preparation of cDNA and qPCR
Gel loading hTERT D9 + HEK293 see codes 21June2010
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Gel
cAMP prec.
X X Invitrogen Marker
(8 μL)
HeLa -S
(20 μL)
HEK293
(20 μL)
Biorad Marker
(8 μL)
C1
(20 μL)
C2
(20 μL)
C3
(20 μL)
S1
(20 μL)
S2
(20 μL)
S3
(20 μL)
X X X
  • Bradford performed by Tina
    • 5,05 mg/mL protein in Lysis buffer
    • 4,02 mg/mL protein in HeLa -S sample

Materials & Methods

Materials

Method

6-wells
# 1 2 3
A
B
24-wells
# 1 2 3 4 5 6
A
B
C
D
96-wells plate
# 01 02 03 04 05 06 07 08 09 10 11 12
A
B
C
D
E
F
G
H
I

Notes

Results

  • Coomassie staining

Discussion

  • DONE Ow..

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