User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/05: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Bradford & cAMP precipitation</span> | ||
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==Summary== | ==Summary== | ||
* | * Bradford (Enspire 2300 & Tecan Safire) | ||
* cAMP precipitation | |||
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==Materials & Methods== | ==Materials & Methods== | ||
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==Notes== | ==Notes== | ||
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==Results== | ==Results== | ||
* | *Sadly no difference could be seen between Enspire and the Tecan reader, and the RIPA buffer (not lysis), still yielded a very high signal | ||
*Everything was put equal to sample S1 and 1 mL of put to 30 μL of 8-AHA-cAMP agarose beads (3x) | |||
==Discussion== | ==Discussion== | ||
* | *Maybe [http://www.piercenet.com/products/browse.cfm?fldID=02020101 Pierce assay] might be a better alternative to the Bradford assay is something in the buffer is interfering | ||
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Revision as of 05:29, 29 July 2010
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 Bradford & cAMP precipitation
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Summary
Results
Discussion
Attachment<html> <a href="http://openwetware.org/wiki/Image:Bradford_05072010.xls"> <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> </a> </html> Bradford results both Tecan and Enspire | |
